Anti-inflammatory mechanism of melanin by the expression of TNS-A, NF-KB in rat liver with Nafld/Nash

Abstract

In the previous study, we have showed the effects of exogenously administered melanin produced by yeast Nadsoniella nigra strain X-1 on the obesity parameters of rats and the development of NAFLD/NASH. It was shown significant decrease of mass indexes and fat accumulation in visceral adipose tissue of treated rats that suggests preventive influence of melanin on obesity. The present study was performed to investigate the anti-inflammatory mechanism of melanin by the expression of TNF-α, NF-κB in rat liver with NAFLD/NASH. There were 30 newborn Wistar male rats, divided into 3 groups: intact (n=10), monosodium glutamate (MSG) (n=10) and MSG + Melanin treated (n=10). Newborns rats of MSG-group and MSG + melanin group received a solution of MSG (4.0 mg/g of body weight) s.c. at 2nd-10th days after birth. Within 4 months after birth, rats had a normal diet. MSG + melanin group received aqueous solution of melanin in dose 1 mg/kg at volume 2.5 ml/kg per os (p.o.).